Des Moines University
2 ARTICLES PUBLISHED IN JoVE
Biology
Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
Victor Babich 1,2, Matthew K. Henry 1, Francesca Di Sole 1
1Physiology and Pharmacology Department, Des Moines University, 2School of Liberal Arts and Sciences, Mercy College of Health Sciences
This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.
Education
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
Wayne A. Wilson 1
1Department of Biochemistry & Nutrition, Des Moines University
Techniques to measure the activity of key enzymes of glycogen metabolism are presented, using a simple spectrophotometer operating in the visible range.
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