Entrar

Des Moines University

2 ARTICLES PUBLISHED IN JoVE

Biology

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Victor Babich^1,2, Matthew K. Henry¹, Francesca Di Sole¹

¹Physiology and Pharmacology Department, Des Moines University, ²School of Liberal Arts and Sciences, Mercy College of Health Sciences

This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.

Education

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Wayne A. Wilson¹

¹Department of Biochemistry & Nutrition, Des Moines University

Techniques to measure the activity of key enzymes of glycogen metabolism are presented, using a simple spectrophotometer operating in the visible range.