Public Health Agency of Canada
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Caroline Gravel *1, Changgui Li *2, Junzhi Wang 2, Anwar M Hashem 1,3,4, Bozena Jaentschke 1, Gary Van Domselaar 5, Runtao He 5, Xuguang Li 1,3
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada
A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.
Immunology and Infection
Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
Ali N. Doucet 1, Carmine J. Slipski 1, George R. Golding 1,2, Michael R. Mulvey 1,2, Denice C. Bay 1
1Department of Medical Microbiology and Infectious Diseases, University of Manitoba, 2National Microbiology Laboratory, Public Health Agency of Canada
This protocol presents methodology to perform biofilm growth and biomass measurements using self-assembled deep well PCR-plate devices for high-throughput 96-well pegged lid static biofilm screening.
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