Le Moyne College

2 ARTICLES PUBLISHED IN JoVE

Behavior

Method to Obtain Pattern of Breathing in Senescent Mice through Unrestrained Barometric Plethysmography

Candace N. Receno¹, Caitlin M. Cunningham², Brianna E. Eassa¹, Rebecca Purdy¹, Lara R. DeRuisseau¹

¹Department of Biological Sciences, Le Moyne College, ²Department of Mathematics, Statistics and Computer Science, Le Moyne College

Unrestrained barometric plethysmography is used to quantify the pattern of breathing in awake mice. We show that 15 s segments under a standardized protocol display similar values to an extended duration of quiet breathing. This methodology also allows for the quantification of apnea and augmented breaths during the first hour in the chamber.

Biology

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Matthew A. Deragon¹, H. John Sharifi², Timothy J. LaRocca¹

¹Department of Life Sciences, Albany College of Pharmacy and Health Sciences, ²Department of Biological and Environmental Sciences, Le Moyne College

As CRISPR-related protocols become increasingly useful and accessible, complications and obstacles can still arise under specific experimental conditions. This protocol outlines the creation of a receptor-interacting serine/threonine-protein kinase 1 (RIPK1/RIP1) knockout human cell line using CRISPR/Cas9 and highlights potential challenges encountered during this process.