Children's Hospital Los Angeles
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Martin Tran 1, Mohit Dave 2,3, Rusty Lansford 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles
We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.
Developmental Biology
Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish
Laila Abd Elmagid *1,2, Nishant Mittal *1,2, Isaac Bakis 1,2, Ching-Ling Lien 3, Michael R. Harrison 1,2
1Cardiovascular Research Institute, Weill Cornell Medicine, 2Department of Cell and Developmental Biology, Weill Cornell Medicine, 3The Saban Research Institute and Heart Institute, Children's Hospital Los Angeles
The present protocol allows efficient and stable delivery of fluorescent microspheres (MS) and quantum dots (QD) into the myocardium of live fish that can be tracked (traced) over time.
