Accedi

Children's Hospital Los Angeles

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

Martin Tran¹, Mohit Dave^2,3, Rusty Lansford^1,2,3

¹Department of Biological Sciences, University of Southern California, Los Angeles, ²Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, ³Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.

Developmental Biology

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

Laila Abd Elmagid^*1,2, Nishant Mittal^*1,2, Isaac Bakis^1,2, Ching-Ling Lien³, Michael R. Harrison^1,2

¹Cardiovascular Research Institute, Weill Cornell Medicine, ²Department of Cell and Developmental Biology, Weill Cornell Medicine, ³The Saban Research Institute and Heart Institute, Children's Hospital Los Angeles

The present protocol allows efficient and stable delivery of fluorescent microspheres (MS) and quantum dots (QD) into the myocardium of live fish that can be tracked (traced) over time.