Kantonsspital Aarau

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models
Fabio Strange 1,2, Sivani Sivanrupan 2, Basil E. Gruter 1,2, Jeannine Rey 2, Dominik Taeschler 2, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research (DBMR), University of Bern

We present a protocol to efficiently evaluate aneurysm perfusion and vessel patency of sidewall aneurysm in rats and rabbits, using fluorescein-based fluorescence video angiography (FVA). With a positive predictive value of 92.6%, it is a simple but very effective and economical method with no special equipment required.

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Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
Stefan Wanderer 1,2, Claudia Waltenspuel 2, Basil E. Grüter 1,2, Fabio Strange 1,2, Sivani Sivanrupan 2, Luca Remonda 3, Hans Rudolf Widmer 4, Daniela Casoni 5, Lukas Andereggen 1,2, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

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Medicine

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit
Gwendoline Boillat 1,2, Tim Franssen 2, Basil Grüter 1,2, Stefan Wanderer 1,2, Kristina Catalano 2, Daniela Casoni 3, Lukas Andereggen 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Experimental Surgery Facility, Department for Biomedical Research, Faculty of Medicine, University of Bern

This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

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Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model
Stefan Wanderer *1,2, Basil E. Grüter *1,2, Jeannine Kümin 1,2, Gwendoline Boillat 1,2, Sivani Sivanrupan 2, Kristina Catalano 1,2, Michael von Gunten 3, Hans Rudolf Widmer 4, Serge Marbacher 1,2,5, Lukas Andereggen 1,2,5
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Institute of Pathology Laenggasse, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

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