Kantonsspital Aarau

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models

Fabio Strange^1,2, Sivani Sivanrupan², Basil E. Gruter^1,2, Jeannine Rey², Dominik Taeschler², Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research (DBMR), University of Bern

We present a protocol to efficiently evaluate aneurysm perfusion and vessel patency of sidewall aneurysm in rats and rabbits, using fluorescein-based fluorescence video angiography (FVA). With a positive predictive value of 92.6%, it is a simple but very effective and economical method with no special equipment required.

Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Stefan Wanderer^1,2, Claudia Waltenspuel², Basil E. Grüter^1,2, Fabio Strange^1,2, Sivani Sivanrupan², Luca Remonda³, Hans Rudolf Widmer⁴, Daniela Casoni⁵, Lukas Andereggen^1,2, Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Department for Biomedical Research, Faculty of Medicine, University of Bern

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

Medicine

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit

Gwendoline Boillat^1,2, Tim Franssen², Basil Grüter^1,2, Stefan Wanderer^1,2, Kristina Catalano², Daniela Casoni³, Lukas Andereggen^1,2, Serge Marbacher^1,2

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Experimental Surgery Facility, Department for Biomedical Research, Faculty of Medicine, University of Bern

This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model

Stefan Wanderer^*1,2, Basil E. Grüter^*1,2, Jeannine Kümin^1,2, Gwendoline Boillat^1,2, Sivani Sivanrupan², Kristina Catalano^1,2, Michael von Gunten³, Hans Rudolf Widmer⁴, Serge Marbacher^1,2,5, Lukas Andereggen^1,2,5

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Institute of Pathology Laenggasse, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.