Kyorin University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

Shinya Urano¹, Ryushi Tazawa¹, Takahito Nei¹, Natsuki Motoi¹, Masato Watanabe², Takenori Igarashi³, Masahiro Tomita³, Koh Nakata¹

¹Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, ²First department of Internal Medicine, School of Medicine, Kyorin University, ³Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd.

We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.

Behavior

Recording Horizontal Saccade Performances Accurately in Neurological Patients Using Electro-oculogram

Yasuo Terao¹, Hideki Fukuda², Yusuke Sugiyama³, Satomi Inomata-Terada¹, Shin-ichi Tokushige⁴, Masashi Hamada³, Yoshikazu Ugawa⁵

¹Department of Cell Physiology, Kyorin University, ²Segawa Memorial Neurological Clinic for Children, ³Department of Neurology, University of Tokyo, ⁴Department of Neurology, Kyorin University, ⁵Department of Neurology, Fukushima Medical University

The article describes a practical method for recording horizontal eye movements with high accuracy by electro-oculogram in neurological patients, using a cup Ag-AgCl electrode with a wide plastic fringe. Stable measurement requires proper selection and fixation of electrodes, taking sufficient time for light adaptation to occur, and re-calibration as needed.