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Kyorin University

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
Shinya Urano 1, Ryushi Tazawa 1, Takahito Nei 1, Natsuki Motoi 1, Masato Watanabe 2, Takenori Igarashi 3, Masahiro Tomita 3, Koh Nakata 1
1Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine, Kyorin University, 3Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd.

We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.

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Behavior

Recording Horizontal Saccade Performances Accurately in Neurological Patients Using Electro-oculogram
Yasuo Terao 1, Hideki Fukuda 2, Yusuke Sugiyama 3, Satomi Inomata-Terada 1, Shin-ichi Tokushige 4, Masashi Hamada 3, Yoshikazu Ugawa 5
1Department of Cell Physiology, Kyorin University, 2Segawa Memorial Neurological Clinic for Children, 3Department of Neurology, University of Tokyo, 4Department of Neurology, Kyorin University, 5Department of Neurology, Fukushima Medical University

The article describes a practical method for recording horizontal eye movements with high accuracy by electro-oculogram in neurological patients, using a cup Ag-AgCl electrode with a wide plastic fringe. Stable measurement requires proper selection and fixation of electrodes, taking sufficient time for light adaptation to occur, and re-calibration as needed.

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