Loyola University Medical Center
2 ARTICLES PUBLISHED IN JoVE
Biology
A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development
Valerie A. Ray 1, Andrew R. Morris 1, Karen L. Visick 1
1Department of Microbiology and Immunology, Loyola University Medical Center
We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.
Biology
Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation
Tyler Compton 1, Nicholas Poellinger 2, Janine Struve 3, John G. Krolikowski 1, James T. Ninomiya 4, Dorothee Weihrauch 1
1Department of Anesthesiology, Medical College of Wisconsin, 2Department of Internal Medicine, Loyola University Medical Center, 3Department of Orthopedic Surgery, Medical College of Wisconsin, 4Department of Orthopedic Surgery, University of New Mexico
We describe the reduction of reperfusion injury by 670 nm irradiation in a mouse model of ischemia and reperfusion by tourniquet placement. This 670 nm irradiation reduced the inflammatory response, decreased the number of proinflammatory macrophages, and increased the protective macrophages.
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