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Loyola University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Biology

A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

Valerie A. Ray¹, Andrew R. Morris¹, Karen L. Visick¹

¹Department of Microbiology and Immunology, Loyola University Medical Center

We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.

Biology

Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation

Tyler Compton¹, Nicholas Poellinger², Janine Struve³, John G. Krolikowski¹, James T. Ninomiya⁴, Dorothee Weihrauch¹

¹Department of Anesthesiology, Medical College of Wisconsin, ²Department of Internal Medicine, Loyola University Medical Center, ³Department of Orthopedic Surgery, Medical College of Wisconsin, ⁴Department of Orthopedic Surgery, University of New Mexico

We describe the reduction of reperfusion injury by 670 nm irradiation in a mouse model of ischemia and reperfusion by tourniquet placement. This 670 nm irradiation reduced the inflammatory response, decreased the number of proinflammatory macrophages, and increased the protective macrophages.