State University of New York Downstate Medical Center
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
Rafael E. Flores-Obando 1, Mona M. Freidin 2, Charles K. Abrams 2
1Program in Molecular and Cellular Biology, State University of New York Downstate Medical Center, 2Department of Neurology and Rehabilitation, University of Illinois at Chicago
We describe the immunomagnetic isolation of primary mouse oligodendrocytes, which allows the rapid and specific isolation of the cells for in vitro culture.
Bioengineering
Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue
Seyed Mohammad Mir 1, Jiawen Chen 1, Meghan R. Pinezich 1, John D. O’Neill 3, Brandon A. Guenthart 4, Gordana Vunjak-Novakovic 2, Jinho Kim 1
1Department of Biomedical Engineering, Stevens Institute of Technology, 2Department of Biomedical Engineering, Columbia University, 3Department of Cell Biology, State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery, Stanford University
The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.
