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Tianjin Medical University

4 ARTICLES PUBLISHED IN JoVE

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Chemistry

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Li Xie 1, Liqin Chen 3, Pan Gu 4, Lanlan Wei 1, Xuejun Kang 2
1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School

We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

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Medicine

Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model
Jaesung P. Choi 1,2, Xi Yang 3, Matthew Foley 4, Xian Wang 1, Xiangjian Zheng 1,2,3
1Lab of Cardiovascular Signaling, Centenary Institute, 2Faculty of Medicine, Sydney Medical School, University of Sydney, 3Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, 4Australian Centre for Microscopy & Microanalysis, University of Sydney

This protocol demonstrates the induction of cerebral cavernous malformation disease in a mouse model and uses contrast enhanced micro computed tomography to measure lesion burden. This method enhances the value of established mouse models to study the molecular basis and potential therapies for cerebral cavernous malformation and other cerebrovascular diseases.

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Medicine

Full-Endoscopic Isolation Zone Technique for the Treatment of Lumbar Disc Herniation
Lu Wang *1, Lingxia Li *2, Cai Cheng 1, Zhiyuan Guo 1, Dasen Xin 1, Xinbiao Yan 1, Weibin Li 1, Hongmin Han 1
1Department of Orthopedics, Cangzhou Central Hospital, Tianjin Medical University, 2Department of Clinical Pharmacy, People's Hospital of Cangzhou City

Here, we present a protocol for the "isolation zone" technique in the treatment of lumbar disc herniation (LDH) under full-endoscopic spine surgery (FESS), including intervertebral foramen formation, targeted catheterization, nucleus pulposus resection, and annulus fibrosus formation, which together completely block the pain from the nerve conduction pathway.

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Biology

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Yuping Fan *1,2, Yan Teng *3, Fu tong Liu *4, Fengxia Ma 1,2, Alan Y. Hsu 5, Sizhou Feng 1,2, Hongbo R. Luo 5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center

This protocol details the preparation of CLON-G to extend the neutrophil lifespan to greater than 5 days and provides a reliable procedure for evaluating neutrophil death with flow cytometry and confocal fluorescence microscopy.

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