University Medical Center

3 ARTICLES PUBLISHED IN JoVE

Biology

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

Meltem Avci-Adali¹, Andreas Behring¹, Heidrun Steinle¹, Timea Keller¹, Stefanie Krajeweski¹, Christian Schlensak¹, Hans P. Wendel¹

¹Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen

In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells.

Bioengineering

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Tatjana Michel¹, Antonia Link¹, Meike-Kristin Abraham^1,2, Christian Schlensak¹, Karlheinz Peter^2,3, Hans-Peter Wendel¹, Xiaowei Wang^2,3, Stefanie Krajewski¹

¹Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, ²Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, ³Department of Medicine, Monash University

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

Medicine

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Antonia Link¹, Giorgio Cattaneo^2,3, Eduard Brynda⁴, Tomas Riedel⁴, Johanka Kucerova⁴, Christian Schlensak¹, Hans Peter Wendel¹, Stefanie Krajewski¹, Tatjana Michel¹

¹Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, ²Acandis GmbH, ³Institute of Biomedical Engineering, University of Stuttgart, ⁴Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic

This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices.