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University Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Biology

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
Meltem Avci-Adali 1, Andreas Behring 1, Heidrun Steinle 1, Timea Keller 1, Stefanie Krajeweski 1, Christian Schlensak 1, Hans P. Wendel 1
1Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen

In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells.

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Bioengineering

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Tatjana Michel 1, Antonia Link 1, Meike-Kristin Abraham 1,2, Christian Schlensak 1, Karlheinz Peter 2,3, Hans-Peter Wendel 1, Xiaowei Wang 2,3, Stefanie Krajewski 1
1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, 2Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, 3Department of Medicine, Monash University

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

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Medicine

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow
Antonia Link 1, Giorgio Cattaneo 2,3, Eduard Brynda 4, Tomas Riedel 4, Johanka Kucerova 4, Christian Schlensak 1, Hans Peter Wendel 1, Stefanie Krajewski 1, Tatjana Michel 1
1Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, 2Acandis GmbH, 3Institute of Biomedical Engineering, University of Stuttgart, 4Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic

This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices.

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