SungKyunKwan University

7 ARTICLES PUBLISHED IN JoVE

Engineering

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Dasol Lee^*1, Minkyung Kim^*1, Sunae So^*1, Inki Kim¹, Gwanho Yoon¹, Kyunghoon Kim², Junsuk Rho^1,3

¹Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), ²School of Mechanical Engineering, Sungkyunkwan University, ³Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)

The use of a hyperlens has been regarded as a novel super-resolution imaging technique due to its advantages in real-time imaging and its simple implementation with conventional optics. Here, we present a protocol describing the fabrication and imaging applications of a spherical hyperlens.

Neuroscience

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Honggun Lee¹, Constantin Radu¹, Jeung Whan Han², Regis Grailhe³

¹Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, ²School of Pharmacy, Sungkyunkwan University, ³Technology Development Platform, Institut Pasteur Korea

We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.

Engineering

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

Healin Im^*1, Seok Hwan Jeong^*1, Dong Hyuk Park², Sunkook Kim¹, Young Ki Hong¹

¹School of Advanced Materials Science and Engineering, Sungkyunkwan University, ²Department of Applied Organic Materials Engineering, Inha University

A protocol for fabricating nanoporous anodic aluminum oxides via simultaneous multi-surfaces anodization followed by stair-like reverse biases detachments is presented. It can be applied repeatedly to the same aluminum substrate, exhibiting a facile, high-yield, and environmentally clean strategy.

Neuroscience

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Junhwan Kwon^1,2, Myunghwan Choi^1,2

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science (IBS)

Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.

Biochemistry

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Jae Gon Kim^*1, Sang Woong Park^*2, Kyung Chul Shin¹, Bokyung Kim¹, Doyoung Byun³, Young Min Bae¹

¹Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, ²Department of Emergency Medical Services, Eulji University, ³Department of Mechanical Engineering, Sungkyunkwan University

Mechanosensitive ion channels are often studied in terms of fluid flow/shear force sensitivity with patch-clamp recording. However, depending on the experimental protocol, the outcome on fluid flow-regulations of ion channels can be erroneous. Here, we provide methods for preventing and correcting such errors with a theoretical basis.

Neuroscience

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

Jisoo Han^1,2, Pyonggang Choi³, Myunghwan Choi³

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science, ³School of Biological Sciences, Seoul National University

The article introduces the µTongue (microfluidics-on-a-tongue) device for functional taste cell imaging in vivo by integrating microfluidics into an intravital imaging window on the tongue.

Chemistry

Preparation of Contiguous Bisaziridines for Regioselective Ring-Opening Reactions

Yeongjin Lee¹, Huimyoung Byeon¹, Hyun-Joon Ha², Jung Woon Yang¹

¹Department of Energy Science, Sungkyunkwan University, ²Department of Chemistry, Hankuk University of Foreign Studies

Contiguous bisaziridines containing non-activated and activated aziridines were synthesized by asymmetric organocatalytic aziridinations and then subjected to chemoselective ring-opening reactions under acidic or basic conditions. The non-activated aziridine ring opens with less reactive nucleophiles under acidic conditions, whereas the activated aziridine ring opens with more reactive nucleophiles under basic conditions.