Maastricht University Medical Center
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Wendy L.J. Hansen 1, Judith Beuving 1, Annelies Verbon 2, Petra. F.G. Wolffs 1
1Department of Medical Microbiology, Maastricht University Medical Center, 2Department of Internal Medicine, Erasmus Medical Center
The design of a straightforward one-day workflow scheme for bacterial pathogen diagnostics enables the rapid recognition of bloodstream infections. The inclusion of eight clinically relevant bacterial targets and their antibiotic resistance profiles offers the clinician an initial insight on the same day, which can lead to more adequate therapy.
Developmental Biology
Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing
Naomi J. M. Kakiailatu 1, Laura E. Kuil 1,2, Eric Bindels 3, Joke T. M. Zink 3, Michael Vermeulen 3, Veerle Melotte 4, Maria M. Alves 1
1Department of Clinical Genetics, Erasmus University Medical Center - Sophia Children's Hospital, 2Division of Psychosocial Research and Epidemiology, the Netherlands Cancer Institute, 3Department of Hematology, Erasmus Medical Center, 4Department of Pathology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Center
Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.
