California State University, Northridge
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
Christopher C. Walheim 1, Juan Pablo Zanin 2, Maria Elena de Bellard 1
1Department of Biology, California State University, Northridge, 2Centro de Biología Celular y Molecular, Universidad Nacional de Córdoba
An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.
Biology
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Faqiang Wu 1, Yoshie Hanzawa 1
1Department of Biology, California State University, Northridge
We developed a simple and efficient protocol for the preparation of large quantities of soybean protoplasts to study complex regulatory and signaling mechanisms in live cells.
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