California State University, Northridge

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

Christopher C. Walheim¹, Juan Pablo Zanin², Maria Elena de Bellard¹

¹Department of Biology, California State University, Northridge, ²Centro de Biología Celular y Molecular, Universidad Nacional de Córdoba

An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

Biology

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Faqiang Wu¹, Yoshie Hanzawa¹

¹Department of Biology, California State University, Northridge

We developed a simple and efficient protocol for the preparation of large quantities of soybean protoplasts to study complex regulatory and signaling mechanisms in live cells.