Johannes Kepler University Linz
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Yong Yu 1, Siu Yee New 1, Jiaxian Lin 2, Xiaodi Su 1, Yen Nee Tan 1
1Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2School of Chemical & Biomedical Engineering, Nanyang Technological University
A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.
Cancer Research
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Eva Dovjak 1, Mario Mairhofer 1, Claudia Wöß 1, Jingjing Qi 1, Dorothy N. Y. Fan 2, Clemens A. Schmitt 1,2,3, Yong Yu 1
1Johannes Kepler University Linz, 2Charité - University Medicine Berlin, 3Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association
Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.
