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Johannes Kepler University Linz

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Yong Yu¹, Siu Yee New¹, Jiaxian Lin², Xiaodi Su¹, Yen Nee Tan¹

¹Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), ²School of Chemical & Biomedical Engineering, Nanyang Technological University

A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.

Cancer Research

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Eva Dovjak¹, Mario Mairhofer¹, Claudia Wöß¹, Jingjing Qi¹, Dorothy N. Y. Fan², Clemens A. Schmitt^1,2,3, Yong Yu¹

¹Johannes Kepler University Linz, ²Charité - University Medicine Berlin, ³Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Here we present a flow cytometry-based method for visualization and quantification of multiple senescence-associated markers in single cells.