Saitama Medical University
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
Susumu Ohshima 1, Atsushi Seyama 2
1Division of Morphological Science, Biomedical Research Center, Saitama Medical University, 2Department of Pathology, International Medical Center, Saitama Medical University
Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
Behavior
A Common Marmoset Model of Mother-Infant Intervention for Breastfeeding Disorders in the Presence of Paternal Inhibition and Maternal Neglect
Mayuko Yoda 1,2, Yoshimasa Kamei 3, Hayato Sakurai 4, Hiroko Kakei 4, Ting Tao 1,2,4, Hideo Yamanouchi 4, Tetsuya Kunikata 4, Masanori Hariyama 5, Ricki Colman 6, Mamiko Koshiba 1,4,5
1Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 2Faculty of Health Sciences and Nursing, Juntendo University, 3Obstetrician, Medical Department, Saitama Medical University, 4Department of Pediatrics, Saitama Medical University, 5Graduate School of Information Sciences, Tohoku University, 6Department of Cell and Regenerative Biology and Wisconsin National Primate Research Center, University of Wisconsin, Madison
Here, we present a nonhuman primate model of mother-infant intervention for breastfeeding disorders in the presence of paternal inhibition and maternal neglect. The mate model video complements education to support primate and human caregivers with respect to infants with breastfeeding problems such as pain.
