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Saitama Medical University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

¹Division of Morphological Science, Biomedical Research Center, Saitama Medical University, ²Department of Pathology, International Medical Center, Saitama Medical University

Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.

Behavior

A Common Marmoset Model of Mother-Infant Intervention for Breastfeeding Disorders in the Presence of Paternal Inhibition and Maternal Neglect

Mayuko Yoda^1,2, Yoshimasa Kamei³, Hayato Sakurai⁴, Hiroko Kakei⁴, Ting Tao^1,2,4, Hideo Yamanouchi⁴, Tetsuya Kunikata⁴, Masanori Hariyama⁵, Ricki Colman⁶, Mamiko Koshiba^1,4,5

¹Graduate School of Sciences and Technology for Innovation, Yamaguchi University, ²Faculty of Health Sciences and Nursing, Juntendo University, ³Obstetrician, Medical Department, Saitama Medical University, ⁴Department of Pediatrics, Saitama Medical University, ⁵Graduate School of Information Sciences, Tohoku University, ⁶Department of Cell and Regenerative Biology and Wisconsin National Primate Research Center, University of Wisconsin, Madison

Here, we present a nonhuman primate model of mother-infant intervention for breastfeeding disorders in the presence of paternal inhibition and maternal neglect. The mate model video complements education to support primate and human caregivers with respect to infants with breastfeeding problems such as pain.