Swansea University
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
Roberto Angelini *1, Silvia Russo *2, Angela Corcelli 2, Simona Lobasso 2
1Medical School, Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro
This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.
Immunology and Infection
Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice
Mark Gurney 1, Luke C. Davies 1,2, Ruth E. Jones 1, Valentina M. Bart 1, Robert H. Jenkins 1, Paul Brennan 1, Philip R. Taylor 1,3, Magdalena A. Czubala 1
1Systems Immunity Research Institute, Division of Infection and Immunity, Cardiff University, 2Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science, Swansea University, 3UK Dementia Research Institute
We demonstrate a step-by-step protocol for the investigation of gene function in peritoneal tissue-resident macrophages in vivo, using lentiviral vectors.
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