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Swansea University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Roberto Angelini^*1, Silvia Russo^*2, Angela Corcelli², Simona Lobasso²

¹Medical School, Swansea University, ²Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro

This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

Immunology and Infection

Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice

Mark Gurney¹, Luke C. Davies^1,2, Ruth E. Jones¹, Valentina M. Bart¹, Robert H. Jenkins¹, Paul Brennan¹, Philip R. Taylor^1,3, Magdalena A. Czubala¹

¹Systems Immunity Research Institute, Division of Infection and Immunity, Cardiff University, ²Biomedical Sciences Unit, Faculty of Medicine, Health and Life Science, Swansea University, ³UK Dementia Research Institute

We demonstrate a step-by-step protocol for the investigation of gene function in peritoneal tissue-resident macrophages in vivo, using lentiviral vectors.