University of South Australia

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

¹School of Pharmacy and Medical Sciences, University of South Australia

Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.

Immunology and Infection

Measuring the 50% Haemolytic Complement (CH₅₀) Activity of Serum

Maurizio Costabile¹

¹School of Pharmacy and Medical Sciences, University of South Australia

The classical pathway is activated by antibody and culminates in target cell lysis. The CH₅₀ assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH₅₀ of a serum sample, the calculations and interpreting of results.

Medicine

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Benson U.W. Lei^1,2, Miko Yamada¹, Van L.T. Hoang³, Paul J. Belt⁴, Mark H. Moore⁵, Lynlee L. Lin², Ross Flewell-Smith¹, Nhung Dang^1,2, Shoko Tomihara², Tarl W. Prow^1,2

¹Future Industries Institute, University of South Australia, ²Faculty of Medicine, University of Queensland, ³Faculty of Health, Queensland University of Technology, ⁴Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, ⁵Australian Craniofacial Unit, Women's and Children's Hospital

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.