University of South Australia

Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.

The classical pathway is activated by antibody and culminates in target cell lysis. The CH₅₀ assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH₅₀ of a serum sample, the calculations and interpreting of results.

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.