Federal University of Santa Catarina

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in Trypanosoma evansi-Infected Mice

Daniel Sérgio Cipriani¹, Gabriela Kaiser Borges², Luiz Claudio Miletti², Amanda Leite Bastos-Pereira^2,3

¹Residency Program in Veterinary Medicine, Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology, Santa Catarina State University, ³Federal University of Santa Catarina

Here, we present a protocol to evaluate nociception in mice infected with Trypanosoma evansi, using the electronic von Frey apparatus as a tool, measuring mechanical thresholds of the hind paw and viscera.

Medicine

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

Leonardo Gonçalves Gomes¹, Lucas de Figueiredo Soveral¹, Izadora Borgmann Frizzo², Thaise Brancher Soncini^1,3, Lívia Budziarek Eslabão¹, Daiana Silva Marcos Maniero¹, Isis Maia Apolinário de Mello¹, Jussara Kasuko Palmeiro², Thaís Cristine Marques Sincero², Oscar Bruna-Romero⁴, Maria Marlene de Souza Pires⁵, Carlos Rodrigo Zárate-Bladés¹

¹Laboratory of Immunoregulation, iREG, Department of Microbiology, Immunology, and Parasitology, Center for Dysbiosis Control, Federal University of Santa Catarina, ²Laboratory of Applied Molecular Microbiology, MIMA, Department of Clinical Analysis, Center for Dysbiosis Control, Federal University of Santa Catarina, ³Carmela Dutra Maternity Hospital, ⁴Laboratory of Applied Immunology, LIA, Department of Microbiology, Immunology, and Parasitology, Center for Dysbiosis Control, Federal University of Santa Catarina, ⁵Laboratory of Metabolism and Nutrition, MenuLab, Department of Pediatrics, Center for Dysbiosis Control, Federal University of Santa Catarina

The article describes a protocol for reconstituting pasteurized donor milk microbiota using the mother's own milk microbiota in practical, real-world settings. It demonstrates effective bacterial growth and microbiome modulation, supporting the feasible application of this procedure within the routine care of a maternity hospital and its associated human milk bank.