huaqiao university

6 ARTICLES PUBLISHED IN JoVE

Biology

In situ Hybridization for Sipunculus nudus Coelomic Fluid

¹Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences, Huaqiao University

This protocol describes an effective in situ hybridization approach to detect the mRNA expression levels and spatial patterns of target genes in Sipunculus nudus coelomic fluid.

Bioengineering

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Sheng-Chang Luo^1,2, Ying Wang³, Ranjith Kumar Kankala^1,2, Yu Shrike Zhang⁴, Ai-Zheng Chen^1,2

¹Institute of Biomaterials and Tissue Engineering, Huaqiao University, ²Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, ³Affiliated Dongguan Hospital, Southern Medical University, ⁴Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School

The present protocol describes the fabrication of poly(lactic-co-glycolic acid)-based highly open porous microspheres (HOPMs) via the single-emulsion formulation based facile microfluidic technology. These microspheres have potential applications in tissue engineering and drug screening.

Biochemistry

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School, Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Here, we present an affinity purification method of a fibrinolytic enzyme from Sipunculus nudus that is simple, inexpensive, and efficient.

Biochemistry

A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots

Wenwu Lin^*1, Hongjun Lin^*2, Pengliang Xin³, Hui Yan², Bin Kang⁴, Mingqing Tang²

¹Emergency Department, Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School, Huaqiao University, ³Department of Haematology, Quanzhou First Hospital Affiliated to Fujian Medical University, ⁴Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University

This study describes a fast and effective method for the cell component analysis of cerebral blood clots through clot dissolving, cell staining, and routine blood examination.

Biochemistry

Rapid Separation and Display of Active Fibrinogenolytic Agents in Sipunculus nudus through Fibrinogen-Polyacrylamide Gel Electrophoresis

Bin Kang^*1, Chengjia Hu^*2, Hongjun Lin², Hui Yan², Chengyun Wei³, Mingqing Tang²

¹Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School, Huaqiao University, ³Department of Laboratory Medicine, Hospital of Huaqiao University

Here, we present a fibrinogen-polyacrylamide gel electrophoresis (PAGE) protocol to rapidly separate and display the fibrinogenolytic agents of Sipunculus nudus.

Genetics

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Dongyang Xu¹, Lu Tang¹, Philipp Kapranov²

¹Institute of Genomics, School of Medicine, Huaqiao University, ²State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University

Insertional mutagenesis is an essential tool in forward genetics for identifying functional genomic elements. Here, we describe the Insertion-based Screen for functional Elements and Transcripts (InSET), a method for detecting lentivirus integration sites within a lentivirus-based insertional mutagenesis cell library.