Universidad Autónoma del Estado de Morelos

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

¹Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, ²Instituto de Biotecnologìa, Universidad Nacional Autónoma de México

We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.

Immunology and Infection

Preparation of CD4⁺ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Tania M. Villanueva-Cabello¹, Iván Martinez-Duncker¹

¹Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular, Universidad Autónoma del Estado de Morelos

We describe a standard antibody staining protocol for use in microscopy to determine the membrane expression and localization of gangliosides in resting and activated human naïve CD4⁺ T cells. Also described are real-time PCR experiments using <40,000 cells that do not require additional low input RNA kits.

Children Touching Words: Studying Tactile Sound Symbolism in Young Children

Alberto Falcón¹, Ulianov Montano¹, Mariel Tavira¹, Osmara Domínguez-Gallegos¹

¹Laboratorio de Comunicación Humana y Cognición, Facultad de Comunicación Humana, Universidad Autónoma del Estado de Morelos

Sound symbolism in the tactile modality has been scarcely studied, and only in adults. This article presents a protocol to study tactile sound symbolism in young children by using play, toys, and touch-appropriate materials. The protocol has been tested with Spanish speaking children.