登录

Emory University, Atlanta, GA

1 ARTICLES PUBLISHED IN JoVE

image

Biology

Imaging the Gut with "CLARITY"
Bindu Chandrasekharan 1, Andrew S. Neish 1
1Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA

We describe the protocol for passive CLARITY (PACT) staining of mouse intestine to enable visualization of subepithelial tissues, including neurons, glia and enteroendocrine cells (EEC) without tissue sectioning. The protocol involves hydrogel embedding of formaldehyde-fixed tissue, and subsequent delipidation using an anionic detergent to "clear" the tissue.

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2025 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。