Imaging the Gut with "CLARITY"Bindu Chandrasekharan 1, Andrew S. Neish 1
1Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA
We describe the protocol for passive CLARITY (PACT) staining of mouse intestine to enable visualization of subepithelial tissues, including neurons, glia and enteroendocrine cells (EEC) without tissue sectioning. The protocol involves hydrogel embedding of formaldehyde-fixed tissue, and subsequent delipidation using an anionic detergent to "clear" the tissue.
