University of Zaragoza
3 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Santiago Uranga 1, Dessislava Marinova 1, Carlos Martin 1, Nacho Aguilo 1
1Department of Microbiology, University of Zaragoza
We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.
Cancer Research
Transmitochondrial Cybrid Generation Using Cancer Cell Lines
Ruth Soler-Agesta 1, Joaquín Marco-Brualla 1, Patricio Fernández-Silva 1,2, Pilar Mozas 1,3, Alberto Anel 1, Raquel Moreno Loshuertos 1,2
1Department of Biochemistry, University of Zaragoza, 2Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, 3Sequencing and Functional Genomics Unit, University of Zaragoza-IACS
This protocol describes a technique for cybrid generation from suspension-growing cancer cells as a tool to study the role of mitochondria in the tumorigenic process.
Biochemistry
Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
Raquel Moreno-Loshuertos 1,2, Patricio Fernández-Silva 1,2
1Department of Biochemistry, University of Zaragoza, 2Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza
This protocol describes a technique for the analysis of respiratory supercomplexes when only small amounts of samples are available.
关于 JoVE
版权所属 © 2024 MyJoVE 公司版权所有，本公司不涉及任何医疗业务和医疗服务。