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University of Zaragoza

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

Santiago Uranga¹, Dessislava Marinova¹, Carlos Martin¹, Nacho Aguilo¹

¹Department of Microbiology, University of Zaragoza

We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.

Cancer Research

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Ruth Soler-Agesta¹, Joaquín Marco-Brualla¹, Patricio Fernández-Silva^1,2, Pilar Mozas^1,3, Alberto Anel¹, Raquel Moreno Loshuertos^1,2

¹Department of Biochemistry, University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, ³Sequencing and Functional Genomics Unit, University of Zaragoza-IACS

This protocol describes a technique for cybrid generation from suspension-growing cancer cells as a tool to study the role of mitochondria in the tumorigenic process.

Biochemistry

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Raquel Moreno-Loshuertos^1,2, Patricio Fernández-Silva^1,2

¹Department of Biochemistry, University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza

This protocol describes a technique for the analysis of respiratory supercomplexes when only small amounts of samples are available.