University of New Mexico Health Sciences Center

4 ARTICLES PUBLISHED IN JoVE

Medicine

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Russell A. Morton¹, Marvin R. Diaz¹, Lauren A. Topper¹, C. Fernando Valenzuela¹

¹Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center

We demonstrate the construction of alcohol vapor chambers using readily available materials that simultaneously house 6 mouse cages. We further describe their use in a mouse model of fetal alcohol exposure equivalent to all 3 trimesters of human pregnancy. This paradigm exposes animals during gestation and postnatal days 1-12.

Biochemistry

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Elizabeth M. Bailey¹, Emanuel Salazar-Cavazos¹, Rachel M. Grattan¹, Michael J. Wester², David J. Schodt², Julian A. Rojo¹, Keith A. Lidke^2,3, Diane S. Lidke^1,3

¹Department of Pathology, University of New Mexico School of Medicine, ²Department of Physics and Astronomy, University of New Mexico, ³Comprehensive Cancer Center, University of New Mexico Health Sciences Center

The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

Behavior

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model

Marena A. Montera¹, Aleyah E. Goins¹, Sascha R. A. Alles¹, Karin N. Westlund¹

¹Department of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center

Methods for simple, rapid induction of a back pain model in mice are provided here using an intraligament injection of urinary plasminogen activator.

Immunology and Infection

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Ziming Cao¹, Matthew J. Garcia², Larry A. Sklar^2,3,4,5, Angela Wandinger-Ness^3,4, Zhichao Fan¹

¹Department of Immunology, School of Medicine, UConn Health, ²Center for Molecular Discovery, University of New Mexico Health Sciences Center, ³Comprehensive Cancer Center, University of New Mexico Health Sciences Center, ⁴Department of Pathology, University of New Mexico Health Sciences Center, ⁵Autophagy, Inflammation, & Metabolism (AIM) Center, University of New Mexico

This protocol describes a flow cytometry-based, high-throughput screening method to identify small-molecule drugs that inhibit β2 integrin activation on human neutrophils.