Teikyo University
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Kotaro Hama 1, Yuko Fujiwara 1, Kazuaki Yokoyama 1
1Faculty of Pharmaceutical Sciences, Teikyo University
Here, we present a protocol to quantify and qualify each sphingomyelin species using multiple reaction monitoring and MS/MS/MS mode, respectively.
Medicine
Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury
Tomohiro Yamaki 1,2, Shinji Onodera 2, Tomoki Uchida 2, Yoshihiro Ozaki 2, Kazuaki Yokoyama 3, Haruko Henmi 2, Mizuho Kamezawa 2, Miyoko Hayakawa 2, Daisuke Itou 1, Nobuo Oka 1,2, Masaru Odaki 1, Yasuo Iwadate 4, Shigeki Kobayashi 1
1Division of Neurosurgery, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, 2Division of PET imaging, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, 3Tokyo Nuclear Services Co. Ltd., 4Department of Neurological Surgery, Graduate School of Medicine, Chiba University
[18F]-fluorodeoxyglucose (FDG) positron emission tomography-computed tomography is useful for studying glucose metabolism related to brain function. Here, we present a protocol for an [18F]FDG tracer set-up and semiquantitative assessment of the region-of-interest analysis for targeted brain areas associated with clinical manifestations in patients with severe traumatic brain injury.
