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Teikyo University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Kotaro Hama¹, Yuko Fujiwara¹, Kazuaki Yokoyama¹

¹Faculty of Pharmaceutical Sciences, Teikyo University

Here, we present a protocol to quantify and qualify each sphingomyelin species using multiple reaction monitoring and MS/MS/MS mode, respectively.

Medicine

Semi-quantitative Assessment Using [¹⁸F]FDG Tracer in Patients with Severe Brain Injury

Tomohiro Yamaki^1,2, Shinji Onodera², Tomoki Uchida², Yoshihiro Ozaki², Kazuaki Yokoyama³, Haruko Henmi², Mizuho Kamezawa², Miyoko Hayakawa², Daisuke Itou¹, Nobuo Oka^1,2, Masaru Odaki¹, Yasuo Iwadate⁴, Shigeki Kobayashi¹

¹Division of Neurosurgery, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, ²Division of PET imaging, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid, ³Tokyo Nuclear Services Co. Ltd., ⁴Department of Neurological Surgery, Graduate School of Medicine, Chiba University

[¹⁸F]-fluorodeoxyglucose (FDG) positron emission tomography-computed tomography is useful for studying glucose metabolism related to brain function. Here, we present a protocol for an [¹⁸F]FDG tracer set-up and semiquantitative assessment of the region-of-interest analysis for targeted brain areas associated with clinical manifestations in patients with severe traumatic brain injury.