St. George's University of London
2 ARTICLES PUBLISHED IN JoVE
Biology
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Shaochong Zhang 1, Axel Nohturfft 1,2
1Department of Molecular and Cell Biology, Harvard, 2Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences, St. George's University of London
Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-GloTM Luciferase Assay System from Promega.
Biology
Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay
Sonia Christou-Savina 1, Philip L. Beales 1, Daniel P. S. Osborn 2
1Genetics and Genomic Medicine, Institute of Child Health, University College London, 2Molecular Cell Science Research Centre, St. George's University of London
The zebrafish is a popular tool to model chronic kidney disease (CKD). However, their small size makes it impossible to evaluate renal function using traditional methods. We describe a fluorescent dye kidney clearance assay1 that allows quantitative analysis of zebrafish kidney function in CKD.
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