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St. George's University of London

2 ARTICLES PUBLISHED IN JoVE

Biology

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

¹Department of Molecular and Cell Biology, Harvard, ²Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences, St. George's University of London

Here, we describe procedures for studying changes in phagocytosis-induced gene expression with a luciferase-based reporter gene approach using the Dual-GloTM Luciferase Assay System from Promega.

Biology

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Sonia Christou-Savina¹, Philip L. Beales¹, Daniel P. S. Osborn²

¹Genetics and Genomic Medicine, Institute of Child Health, University College London, ²Molecular Cell Science Research Centre, St. George's University of London

The zebrafish is a popular tool to model chronic kidney disease (CKD). However, their small size makes it impossible to evaluate renal function using traditional methods. We describe a fluorescent dye kidney clearance assay¹ that allows quantitative analysis of zebrafish kidney function in CKD.