School of Dentistry, UCLA
2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
Use of Human Perivascular Stem Cells for Bone Regeneration
Aaron W. James *1, Janette N. Zara *2, Mirko Corselli 2, Michael Chiang 1, Wei Yuan 2, Virginia Nguyen 1, Asal Askarinam 1, Raghav Goyal 1, Ronald K. Siu 3, Victoria Scott 1, Min Lee 3, Kang Ting 1, Bruno Péault 2,4, Chia Soo 2
1Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, 2UCLA and Orthopaedic Hospital, Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA, 3Department of Bioengineering, UCLA, 4Center for Cardiovascular Science, University of Edinburgh
Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.
Bioengineering
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
Raymond Wing Moon Lam 1, Sunny Akogwu Abbah 2, Wang Ming 1, Mathanapriya Naidu 1, Felly Ng 1, Hu Tao 1, James Goh Cho Hong 3,4, Kang Ting 5, Wong Hee Kit 1,4
1Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 2Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, 3Department of Bioengineering, Faculty of Engineering, National University of Singapore, 4Tissue Engineering Program, National University of Singapore, 5Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles
Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.
