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School of Dentistry, UCLA

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Use of Human Perivascular Stem Cells for Bone Regeneration

Aaron W. James^*1, Janette N. Zara^*2, Mirko Corselli², Michael Chiang¹, Wei Yuan², Virginia Nguyen¹, Asal Askarinam¹, Raghav Goyal¹, Ronald K. Siu³, Victoria Scott¹, Min Lee³, Kang Ting¹, Bruno Péault^2,4, Chia Soo²

¹Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, UCLA, ²UCLA and Orthopaedic Hospital, Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, UCLA, ³Department of Bioengineering, UCLA, ⁴Center for Cardiovascular Science, University of Edinburgh

Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.

Bioengineering

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Raymond Wing Moon Lam¹, Sunny Akogwu Abbah², Wang Ming¹, Mathanapriya Naidu¹, Felly Ng¹, Hu Tao¹, James Goh Cho Hong^3,4, Kang Ting⁵, Wong Hee Kit^1,4

¹Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, ²Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, ³Department of Bioengineering, Faculty of Engineering, National University of Singapore, ⁴Tissue Engineering Program, National University of Singapore, ⁵Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles

Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.