Tokyo Medical University

4 ARTICLES PUBLISHED IN JoVE

Biology

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Ke Ren¹, Yidan Dai¹, Kai Yi², Masanobu Kinoshita¹, Masahiro Itoh², Ichiro Sakata³, Takafumi Sakai³, Shuang-Qin Yi¹

¹Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, ²Department of Anatomy, Tokyo Medical University, ³Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University

A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.

Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Eriko Katsuta¹, Masanori Oshi¹, Omar M. Rashid^2,3,4,5, Kazuaki Takabe^1,6,7,8,9,10

¹Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, ²Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, ³Department of Surgery, Massachusetts General Hospital, ⁴Department of Surgery, University of Miami Miller School of Medicine, ⁵Department of Surgery, Nova Southeastern University School of Medicine, ⁶Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, ⁷Department of Breast Surgery and Oncology, Tokyo Medical University, ⁸Department of Surgery, Yokohama City University, ⁹Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, ¹⁰Department of Surgery, Fukushima Medical University

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

Medicine

Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis

Toshihiko Isaji^1,2,3, Shun Ono^1,2,4,5, Takuya Hashimoto^1,2,3, Kota Yamamoto^1,2,3, Ryosuke Taniguchi^1,2,3, Haidi Hu^1,2, Tun Wang^1,2, Jun Koizumi⁴, Toshiya Nishibe⁵, Katsuyuki Hoshina³, Alan Dardik^1,2,6

¹Department of Surgery, Yale University, ²Vascular Biology and Therapeutics Program, Yale University, ³Department of Vascular Surgery, University of Tokyo, ⁴Department of Diagnostic Radiology, Tokai University School of Medicine, ⁵Department of Cardiovascular Surgery, Tokyo Medical University, ⁶Department of Vascular Surgery, VA Connecticut Healthcare Systems

An aortocaval fistula was created by puncturing the murine infra-renal aorta through both walls into the inferior vena cava and was followed by creation of a stenosis in its outflow via partial ligation of the inferior vena cava. This reproducible model can be used to study central venous stenosis.

Neuroscience

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Kazuhide Asakawa¹, Hiroshi Handa¹, Koichi Kawakami^2,3

¹Department of Chemical Biology, Tokyo Medical University, ²Division of Molecular and Developmental Biology, National Institute of Genetics, ³Department of Genetics, Graduate University for Advanced Studies (SOKENDAI)

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model.