University of Western Sydney, NSW Australia

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

Antonio Lauto¹, Marcus Stoodley², Matthew Barton¹, John W. Morley¹, David A. Mahns¹, Leonardo Longo³, Damia Mawad¹

¹Bioelectronics and Neuroscience (BENS) research group, University of Western Sydney, NSW Australia, ²Australian School of Advanced Medicine, Macquarie University, NSW Australia, ³School of Medicine, University of Siena, Italy

Sutures are usually needed to repair tissue during surgical procedures. However, their application can be problematic as they are invasive and may damage tissue. The fabrication and application methods of a novel tissue adhesive are here reported. This adhesive film is laser-activated and does not require the use of sutures.

Neuroscience

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

Morven A. Cameron¹, Orsolya Kekesi^1,2, John W. Morley^1,2, Alba Bellot-Saez^1,2, Sindy Kueh², Paul Breen¹, André van Schaik¹, Jonathan Tapson¹, Yossi Buskila^1,2

¹The MARCS Institute, Western Sydney University, ²School of Medicine, Western Sydney University

Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.