Children's Health Research Institute
2 ARTICLES PUBLISHED IN JoVE
Biology
Single Oocyte Bisulfite Mutagenesis
Michelle M. Denomme 1,2,3, Liyue Zhang 3, Mellissa R.W. Mann 1,2,3
1Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Children's Health Research Institute
Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.
Biology
Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
Paul William Dyce 1,2
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, The University of Western Ontario, 2Children's Health Research Institute
In recent years the formation of germ cells using in vitro culture methods has been demonstrated using several different types of somatic stem cells. This article will visually present the differentiation of newborn mouse derived stem cells to early oocyte-like cells.
