Accedi

Children's Health Research Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Michelle M. Denomme^1,2,3, Liyue Zhang³, Mellissa R.W. Mann^1,2,3

¹Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, ²Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, ³Children's Health Research Institute

Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.

Biology

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Paul William Dyce^1,2

¹Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, The University of Western Ontario, ²Children's Health Research Institute

In recent years the formation of germ cells using in vitro culture methods has been demonstrated using several different types of somatic stem cells. This article will visually present the differentiation of newborn mouse derived stem cells to early oocyte-like cells.