Western General Hospital
2 ARTICLES PUBLISHED IN JoVE
Medicine
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Fiach C. O'Mahony 1, Jyoti Nanda 1, Alexander Laird 1, Peter Mullen 2, Helen Caldwell 3, Ian M. Overton 4, Lel Eory 4, Marie O'Donnell 1,5, Dana Faratian 6, Thomas Powles 7, David J. Harrison 1,2, Grant D. Stewart 1
1Edinburgh Urological Cancer Group, University of Edinburgh, 2School of Medicine, University of St Andrews, 3Division of Pathology, University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, 5Department of Pathology, Western General Hospital, 6Breakthrough Breast Cancer Research Unit, University of Edinburgh, 7St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London
RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma.
Bioengineering
Cell Patterning on Photolithographically Defined
Parylene-C: SiO2 Substrates
Mark A. Hughes 1, Paul M. Brennan 2, Andrew S. Bunting 3, Mike J. Shipston 1, Alan F. Murray 3
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh
This protocol describes a microfabrication-compatible method for cell patterning on SiO2. A predefined parylene-C design is photolithographically printed on SiO2 wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO2 regions.
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