Accedi

Western General Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

Fiach C. O'Mahony¹, Jyoti Nanda¹, Alexander Laird¹, Peter Mullen², Helen Caldwell³, Ian M. Overton⁴, Lel Eory⁴, Marie O'Donnell^1,5, Dana Faratian⁶, Thomas Powles⁷, David J. Harrison^1,2, Grant D. Stewart¹

¹Edinburgh Urological Cancer Group, University of Edinburgh, ²School of Medicine, University of St Andrews, ³Division of Pathology, University of Edinburgh, ⁴MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, ⁵Department of Pathology, Western General Hospital, ⁶Breakthrough Breast Cancer Research Unit, University of Edinburgh, ⁷St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London

RPPA enables the protein expression of hundreds of samples, printed on nitrocellulose slides to be interrogated simultaneously, using fluorescently labelled antibodies. This technique has been applied to study the effect of drug treatment heterogeneity within clear cell renal carcinoma.

Bioengineering

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Mark A. Hughes¹, Paul M. Brennan², Andrew S. Bunting³, Mike J. Shipston¹, Alan F. Murray³

¹Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, ²Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, ³School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh

This protocol describes a microfabrication-compatible method for cell patterning on SiO₂. A predefined parylene-C design is photolithographically printed on SiO₂ wafers. Following incubation with serum (or other activation solution) cells adhere specifically to (and grow according to the conformity of) underlying parylene-C, whilst being repulsed by SiO₂ regions.