Janssen Research and Development
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Christina D. King 1, Joseph D. Dudenhoeffer 1, Liqing Gu 2, Adam R. Evans 3, Renã A. S. Robinson 1
1Department of Chemistry, University of Pittsburgh, 2SGS North America Inc., 3Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development
Combined precursor isotopic labeling and isobaric tagging (cPILOT) is a quantitative proteomics strategy that enhances sample multiplexing capabilities of isobaric tags. This protocol describes the application of cPILOT to tissues from an Alzheimer's disease mouse model and wild-type controls.
Chemistry
Easy Access to Aliphatic Sulfonamides using Sulfamoyl Chlorides Under Visible Light Activation
Sandrine M. Hell 1, Claudio F. Meyer 1,2, Andrés A. Trabanco 2, Véronique Gouverneur 1
1Chemistry Research Laboratory, University of Oxford, 2Discovery Chemistry, Janssen Research and Development
Presented here is a protocol for the easy synthesis of aliphatic sulfonamides using sulfamoyl chlorides, (TMS)3SiH and Eosin Y under blue-light irradiation.
