Accedi

Janssen Research and Development

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Christina D. King¹, Joseph D. Dudenhoeffer¹, Liqing Gu², Adam R. Evans³, Renã A. S. Robinson¹

¹Department of Chemistry, University of Pittsburgh, ²SGS North America Inc., ³Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science, Janssen Research and Development

Combined precursor isotopic labeling and isobaric tagging (cPILOT) is a quantitative proteomics strategy that enhances sample multiplexing capabilities of isobaric tags. This protocol describes the application of cPILOT to tissues from an Alzheimer's disease mouse model and wild-type controls.

Chemistry

Easy Access to Aliphatic Sulfonamides using Sulfamoyl Chlorides Under Visible Light Activation

Sandrine M. Hell¹, Claudio F. Meyer^1,2, Andrés A. Trabanco², Véronique Gouverneur¹

¹Chemistry Research Laboratory, University of Oxford, ²Discovery Chemistry, Janssen Research and Development

Presented here is a protocol for the easy synthesis of aliphatic sulfonamides using sulfamoyl chlorides, (TMS)₃SiH and Eosin Y under blue-light irradiation.